치주세균에 감염된 구강암 세포 Ca9-22에서 유래한 exosomal small RNA 특성 및 기능 분석

유연이; 유미라; 김다정; 김윤학; 박혜련

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치주세균에 감염된 구강암 세포 Ca9-22에서 유래한 exosomal small RNA 특성 및 기능 분석
Analysis on Exosomal Small RNA Derived from Periodontal Pathogen-infected Ca9-22 Oral Cancer Cells

대한구강악안면병리학회지 2022년 46권 5호 p.83 ~ 90

유연이, 유미라, 김다정, 김윤학, 박혜련,

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유연이 ( Yu Yeun-I ) - Pusan National University School of Medicine Biomedical Research Institute
유미라 ( Yu Mi-Ra ) - Pusan National University School of Dentistry Department of Oral Pathology
김다정 ( Kim Da-Jeong ) - Pusan National University School of Dentistry Department of Oral Pathology
김윤학 ( Kim Yun-Hak ) - Pusan National University School of Medicine Department of Anatomy
박혜련 ( Park Hae-Ryoun ) - Pusan National University School of Dentistry Department of Oral Pathology

KMID : 0829220220460050083 DOI : 10.17779/KAOMP.2022.46.5.002

Abstract

Porphyromonas gingivalis, a major pathogen of chronic periodontitis, colonizes in subgingival crevice and affects surrounding oral tissues, especially in periodontitis patients. Oral cancer mainly occurs in old-aged persons, and are exposed to the P. gingivalis, released from periodontitis, one of the most common inflammatory disease of oral cavity. Thus oral cancer cells may be infected with P. gingivalis, and its biologic behavior are autologously and/or heterogeneously modulated by altering gene expression. Exosomes which are derived from cells contain not only coding genes but also non-coding RNAs such as long non-coding RNAs, miRNA, and piRNAs. Here, to investigate the effect of P. gingivalis on oral cancer cells and to gain insight into the crosstalk between inflammatory signal from tumor microenvironment and oral cancer, we observed miRNA profiles of exosomes from P. gingivalis?infected oral cancer cells. Upregulation of 6 miRNAs, miR-203-3p, miR-6516-3p, miR-483-5p, miR-1275, miR-8485, and miR-19a-3p, were observed whereas 14 miRNAs including let-7a-3p, miR-106a-5p were downregulated. In addition, KEGG pathway analysis using the upregulated- and downregulated- miRNAs showed association with cell adhesion molecules pathway and ECM-receptor interaction pathway, respectively. These findings suggest that P. gingivalis could modulate biologic behavior of oral cancer cells through changes of exosomal miRNAs.